Abstract
ABSTRACT: Acute imbalance between elastase and α-1- proteinase inhibitor (α1Pi) may contribute to the development of bronchopulmonary dysplasia (BPD). The question of whether such an imbalance persists in BPD infants still requiring mechanical ventilation after 4 wk of life has not been previously addressed. We studied 14 infants still on mechanical ventilation at 4 wk of age: nine had BPD and five did not. Weekly (4 to 9 wk) serum and bronchoalveolar lavage (BAL) specimens were taken. αlPi and α-2-macroglobulin were measured in serum and BAL by immunoturbidimetric assay. BAL elastase activity was measured by cleavage of a synthetic substrate and expressed as ng of porcine pancreatic elastase equivalent. Infants with BPD had higher levels of serum αlPi and α-2-macroglobulin than those without BPD. In contrast, the corresponding BAL levels were either similar or even decreased (α1Pi). Moreover, there was a 3-fold increase in elastase-1Pi imbalance expressed as the BAL ng of porcine pancreatic elastase equivalent/2αlPi ratio. The role of nosocomial infections was evident in a subgroup of 11 infected BAL aspirates in BPD infants. In such cases we found a 3-fold increase in the BAL ng of porcine pancreatic elastase equivalent/αlPi ratio as compared to 35 noninfected BAL in BPD infants. These data suggest a persistent alveolitis with imbalance between elastase and proteinase inhibitors in prolonged severe BPD. Such an imbalance is, in part, explained by a local destruction and/or inactivation of αlPi. Our results also emphasize the increase in proteolysis with nosocomial pneumonia.
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Walti, H., Tordet, C., Gerbaut, L. et al. Persistent Elastase/Proteinase Inhibitor Imbalance during Prolonged Ventilation of Infants with Bronchopulmonary Dysplasia: Evidence for the Role of Nosocomial Infections. Pediatr Res 26, 351–355 (1989). https://doi.org/10.1203/00006450-198910000-00013
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DOI: https://doi.org/10.1203/00006450-198910000-00013
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