Abstract
Monocytes (M) and neutrophils (N) are implicated in hyperoxic lung injury. We investigated effects of surfactant (ST) on N and M in hyperoxia (HO2). METHODS: N and M from healthy adults were plated at 1×106-4×106/ml. Cells were incubated with (+ST) or without (-ST) surfactant (100μg/ml;Exosurf) and exposed to either HO2 (95% O2+5% CO2) or air, for 2 to 18 hours. Supernatants was examined for TNF-α: and IL-8 (ELISA). Cells were examined for PMA stimulated Oxygen Radical (OR) production by luminol dependent chemiluminescence. RESULTS: i)By 2 hours HO2 increased OR by N; ST reduced OR, both in air and HO2 (3 subjects). Peak OR (mV) as (Mean(SD).
In N IL-8 release was not signficantly affected by ST.
CONCLUSIONS:ST significantly downregulates TNF-α and OR release by N and M. This may be of potential importance.
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Kirpalani, H., Conlon, P. & Jordana, H. 108 SURFACTANT DOWNREGULATES HUMAN MONOCYTE AND NEUTROPHIL RESPONSES TO HYPEROXIA. Pediatr Res 36, 20 (1994). https://doi.org/10.1203/00006450-199407000-00108
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DOI: https://doi.org/10.1203/00006450-199407000-00108
