190 PCBS, PCDDS AND PCDFS IN HUMAN MILK DOES A SHORT TERM-CHANGE IN MATERNAL DIET INFLUENCE THEIR CONCENTRATION?

Abstract

INTRODUCTION: Polychlorinated Biphenyls (PCBs), Dibenzodioxins (PCDDs) and -Furans (PCDFs) are found mainly in animal fat and in human milk.

OBJECTIVES: To elucidate the influence of a short-term change in maternal diet in the PCB, PCDD and PCDF content of human milk.

STUDY GROUP: 6 breastfeeding mothers of healthy, term, single infants 3-10 months old. Diet: 24-hours fasting and weekly change of a weighed recorded diet containing high (“HIGH”) or extremely low (“LOW”) fat, cross-over design. Samples: 1 human milk sample before the study (“start-up”), 1 sample after fasting (“fasting”) and 1 sample at the end of every diet week (“HIGH” and “LOW”) in pre-washed bottles (WHO protocoll), stored at −20° until analysis.

METHODS: Lipid extraction using polyethylene film dialysis and silica column, HPLC- separation of the congeners, analysis on High- resolution gas chromatograph (HP-5898) and a high- resolution- mass spectrometer (VG-70-250S).

STATISTICAL ANALYSIS: Latin- square test.

RESULTS: Mean animal fat intake of the mothers during “LOW” weeks 9.13 (9.65)g/day vs 108.04 (56.46)g/day during “HIGH” weeks.(p<0.01)

Latin-square analysis: PCBs, PCDDS, PCDFs: No influence of dietary changes.

CONCLUSION: No dietary changes for breastfeeding mothers.

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