Deletions in mitochondrial DNA (mt-DNA) during hypoxic cell death in the human NT2-N neuronal cell line

Background: Mitochondrial damage and delayed cellular energy failure may be a cause of hypoxic cell death. Mt-DNA deletions are seen with ageing and in some neuro-degenerative diseases and may contribute to cell death.

Materials and methods: Human NT2-N neurons were exposed to hypoxia for 6 hours. Common deletion PCR (primers 8273-8305 and 13720-13692) and long PCR (primers 3470-3492 and 16379-16360) were performed after 48 hours when marked cell death was apparent. Nested PCR and Southern blots were done to verify the origin of the products.

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