Abstract
We investigated whether levels of serum collagen markers and serum osteocalcin are related to pubertal growth and development in a cross-sectional study of 57 healthy boys at 14 y of age. The level of the soft tissue marker, serum amino-terminal propeptide of type III procollagen(PIIINP) was higher in boys at Tanner stages G3 versus G2(p < 0.01). The levels of the markers of bone collagen matrix differed only at a more advanced pubertal stage: the formation markers, carboxy-terminal and amino-terminal propeptides of type I procollagen, and the degradation marker, carboxy-terminal telopeptide of type I collagen were higher only at stage G4 versus G3 (p < 0.01). The marker of bone mineralization, serum osteocalcin was also higher only at stage G4 versus G3 (p < 0.01). Stage G4 was associated with the pubertal growth spurt. The results demonstrate that pubertal development should be taken into account when serum levels of collagen markers and osteocalcin are evaluated, and suggest that an increase in serum PIIINP in boys at G3 might predict a normal pubertal growth spurt, but the finding remains to be confirmed in longitudinal studies.
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Abbreviations
- ICTP:
-
carboxy-terminal telopeptide of type I collagen
- G:
-
Tanner's genital stage
- OC:
-
osteocalcin
- PICP:
-
carboxy-terminal propeptide of type I procollagen
- PIIINP:
-
amino-terminal propeptide of type III procollagen
- PINP:
-
amino-terminal propeptide of type I procollagen
References
Risteli L, Risteli J 1993 Biochemical markers of bone metabolism. Ann Med 25: 385–293.
Charles P, Hasling C, Risteli L, Risteli J, Mosekilde L, Eriksen EF 1992 Assessment of bone formation by biochemical markers in metabolic bone disease: separation between osteoblastic activity at the cell and tissue level. Calcif Tissue Int 51: 406–411.
Saggese G, Bertelloni Baroncelli GI, DiNero G 1992 Serum levels of carboxyterminal propeptide of type I procollagen in healthy children from the 1st year of life to adulthood and in metabolic bone diseases. Eur J Pediatr 151: 764–768.
Trivedi P, Risteli J, Risteli L, Hindmarsh PC, Brook CGD, Mowat AP 1991 Serum concentrations of the type I and III procollagen propeptides as biochemical markers of growth velocity in healthy infants and children and in children with growth disorders. Pediatr Res 30: 276–280.
Danne T, Gruters A, Schuppan D, Quantas N, Enders I, Weber B 1989 Relationship of procollagen type III propeptide-related antigens in serum to somatic growth in healthy children and patients with growth disorders. J Pediatr 114: 257–260.
Trivedi P, Hindmarsh P, Risteli J, Risteli L, Mowat A, Brook CGD 1989 Growth velocity, growth hormone therapy, and serum concentrations of the amino-terminal propeptide of type III procollagen. J Pediatr 114: 225–230.
Lindqvist U, Laurent TC 1992 Serum hyaluronan and aminoterminal propeptide of type III procollagen: variation with age. Scand J Clin Lab Invest 52: 613–621.
Hertel NT, Stoltenberg M, Juul A, Main KM, Muller J, Nielsen CT, Lorentzen I, Skakkebaek NE 1993 Serum concentrations of type I and type III procollagen propeptides in healthy children and in girls with central precocious puberty during treatment with gonadotropin-releasing hormone analog and cyproterone acetate. J Clin Endocrinol Metab 76: 924–927.
Anttila R, Koistinen R, Seppälä M, Koistinen H, Siimes MA 1994 Insulin-like growth factor I and insulin-like growth factor binding protein 3 as determinants of blood hemoglobin concentration in healthy subjects. Pediatr Res 36: 745–748.
Hansen P, With TK 1952 Clinical measurements of testes. Acta Med Scand Suppl 206: 457–465.
Tanner JM, Whitehouse RH 1965 Clinical longitudinal standards for height, weight, height velocity, weight velocity and stages of puberty. Arch Dis Child 51: 170–179.
Apter D, Jänne O, Karvonen P, Vihko R 1976 Simultaneous determination of five sex hormones in human serum by radioimmunoassay after chromatography on Lipidex-5000. Clin Chem 22: 32–38.
Melkko J, Niemi S, Risteli L, Risteli J 1990 Radioimmunoassay of the carboxyterminal propeptide of human type I procollagen. Clin Chem 36: 1328–1332.
Risteli J, Niemi S, Trivedi P, Mäentausta O, Mowat AP, Risteli L 1988 Rapid equilibrium radioimmunoassay for the aminoterminal propeptide of human type III procollagen. Clin Chem 34: 715–718.
Risteli J, Elomaa I, Niemi S, Novamo A, Risteli L 1993 Radioimmunoassay for the pyridinoline cross-linked carboxy-terminal telopeptide in metabolic bone disease: correlation with bone of type I collagen: a new serum marker of bone collagen degradation. Clin Chem 39: 635–640.
Jackson JA, Kleerekoper M, Parfitt AM, Rao DS, Villenueva AR, Frame B 1987 Bone histomorphometry in hypogonadal and eugonadal men with spinal osteoporosis. J Clin Endocrinol Metab 65: 53–58.
Vanderschueren D, Bouillon R 1995 Androgens and bone. Calcif Tissue Int 56: 341–346.
Prockop DJ, Kivirikko KI, Tuderman L, Guzman NA 1979 The biosynthesis of collagen and its disorders. N Engl J Med 301: 77–85.
Prockop DJ, Kivirikko KI, Tuderman L, Guzman NA 1979 The biosynthesis of collagen and its disorders. N Engl J Med 301: 13–23.
Eriksen EF, Charles P, Melsen F, Mosekilde L, Risteli L 1993 Serum markers of type I collagen formation and degradation in metabolic bone disease: correlation with histomorphometry. J Bone Miner Dis 8: 127–132.
Smedsrod B, Melkko J, Risteli L, Risteli J 1990 Circulating C-terminal propeptide of type I procollagen is cleared mainly via the mannose receptor in liver endothelial cells. Biochem J 271: 345–350.
Melkko J, Hellevik T, Risteli L, Risteli J, Smedsrod B 1994 Clearance of NH2-terminal propeptides of type I and III procollagen is a physiological function of the scavenger receptor in liver endothelial cells. J Exp Med 179: 405–412.
Cowan HB, Vick S, Conary JT, Shepherd VL 1992 Dexamethasone up-regulates mannose receptor activity by increasing mRNA levels. Arch Biochem Biophys 296: 314–320.
Hansen M, Stoltenberg M, Host NB, Lorenzen I, Bentsen KD 1995 Glucocorticoids inhibit the synthesis of type III collagen, but do not affect the hepatic clearance of its aminoterminal propeptide (PIIINP). Scand J Clin Lab Invest 55: 543–548.
Blumsohn A, Hannon RA, Wrate R, Barton J, Al-Dehaimi AW, Colwell A, Eastell R 1994 Biochemical markers of bone turnover in girls during puberty. Clin Endocrinol 40: 663–670.
Price PA, Parthermore JG, Deftos LJ 1980 New biochemical marker for bone metabolism. Measurement by radioimmunoassay of bone GLA protein in the plasma of normal subjects and patients with bone disease. J Clin Invest 66: 878–883.
Delmas PD 1992 Clinical use of biochemical markers of bone remodeling in osteoporosis. Bone Suppl 13: 17–22.
Tarallo P, Henny J, Fournier B, Siest G 1990 Plasma osteocalcin: biological variations and reference limits. Scand J Lab Invest 50: 649–655.
Sidenius Johansen J, Giwercman A, Hartwell D, Nielsen CT, Price PA, Christiansen C 1988 Serum bone GLA-protein as a marker of bone growth in children and adolescents: correlation with age, height, serum insulin-like growth factor I, and serum testosterone. J Clin Endocrinol Metab 67: 273–278.
Lian JB, Gundberg CM 1988 Osteocalcin. Biochemical considerations and clinical applications. Clin Orthop Res 226: 267–280.
Magnusson P, Häger A, Larsson L 1995 Serum osteocalcin and bone and liver alkaline phosphatase isoforms in healthy children and adolescents. Pediatr Res 38: 955–961.
Gundberg CM, Lian JB, Gallop PM 1983 Measurements ofγ-carboxyglutamate and circulating osteocalcin in normal children and adults. Clin Chim Acta 128: 1–8.
Bertelloni S, Baroncelli GI, Battini R, Perri G, Saggese G 1995 Short-term effect of testosterone treatment on reduced bone density in boys with constitutional delay of puberty. J Bone Miner Res 10: 1488–1495.
Finkelstein JS, Neer RM, Biller BMK, Crawford JD, Klibanski A 1992 Osteopenia in men with a history of delayed puberty. N Engl J Med 326: 600–604.
Shaw NJ, Bishop NJ 1995 Mineral accretion in growing bones-a framework for the future. Arch Dis Child 72: 177–179.
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Supported by the Emil Aaltonen Foundation, Tampere, Finland.
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Sorva, R., Anttila, R., Siimes, M. et al. Serum Markers of Collagen Metabolism and Serum Osteocalcin in Relation to Pubertal Development in 57 Boys at 14 Years of Age. Pediatr Res 42, 528–532 (1997). https://doi.org/10.1203/00006450-199710000-00018
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DOI: https://doi.org/10.1203/00006450-199710000-00018
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