Abstract
The dynamic changes of plasma acylcarnitine levels in 1- to 7-y-old children during fasting and after the ingestion of sunflower oil were studied. Glucose, 3-hydroxybutyrate, acetoacetate, FFA, and individual plasma acylcarnitine levels were monitored in both conditions. Fasting experiments lasted for 20 h, and acylcarnitine concentrations were analyzed at 0, 15, and 20 h of fasting. During the fat load, acylcarnitine levels were analyzed at 0, 60, 120, and 180 min. In both tests, a generalized increase of all plasma straight-chain acylcarnitines was observed. Acetylcarnitine contributed the most to the increase of total esterified carnitine. In addition, we demonstrated that the relative increase of each individual acylcarnitine during enhanced fatty acid oxidation is tightly related to its molecular structure and chain length. Fasting as well as the fat load primarily resulted in an increase of unsaturated acylcarnitines. During fasting, C12:1 and C14:1 showed a relatively high increase, whereas after the fat load C16:2 and C14:2, metabolites of linoleic acid (66% of the fat load), were the main acylcarnitines that increased.
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Abbreviations
- CoA:
-
coenzyme A
- LCT:
-
long-chain triglyceride
- KB:
-
ketone bodies
- Glu:
-
glucose
- AcAc:
-
acetoacetate
- 3OHB:
-
3-hydroxybutyrate
- MCT:
-
medium-chain triglyceride
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Supported in part by a grant (BD 2589/93-ID) of Junta Nacional de Investigação Científica e Tecnológica-Programa Praxis XXI (Lisbon, Portugal) to Catarina C. G. Costa.
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Costa, C., De Almeida, I., Jakobs, C. et al. Dynamic Changes of Plasma Acylcarnitine Levels Induced by Fasting and Sunflower Oil Challenge Test in Children. Pediatr Res 46, 440 (1999). https://doi.org/10.1203/00006450-199910000-00013
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DOI: https://doi.org/10.1203/00006450-199910000-00013
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