Abstract
We have previously demonstrated that pressure-overload hypertrophy in adult sheep is associated with myocardial dysfunction whereas that in young lambs is associated with normal contractility. To probe for possible mechanisms of these age-dependent differences, we assessed mRNA expression of genes encoding critical components of myocardial Ca2+ handling in the same animal model. We studied left ventricular myocardium of young and adult sheep with short-term (48 h) and long-term (6 wk) pressure overload induced by ascending aortic constriction. Six weeks of pressure overload induced the significant left ventricular hypertrophy (36 and 39% increase in left ventricular/body weight ratio in lambs and sheep, respectively). The Ca2+ ATPase and Na+/Ca2+ exchanger mRNA decreased with pressure overload only in the adult (p < 0.05). Ca2+ channel mRNA was slightly increased by pressure overload regardless of age (p < 0.05). Calsequestrin, sarcoplasmic reticulum Ca2+ release channel, or myosin heavy-chain mRNA levels did not significantly differ. In adult sheep after 6 wk of pressure overload, decreases in load-adjusted midwall shortening (systolic dysfunction) and prolongation of relaxation time constant (diastolic dysfunction) correlated with decreases in Ca2+-ATPase mRNA. The sarcoplasmic reticulum Ca2+-ATPase protein level and Ca2+ uptake activity of isolated sarcoplasmic reticulum vesicles were depressed only in the adult with pressure-overload hypertrophy but not in the young. We demonstrated age-dependent differences in mRNA expression of Ca2+-handling protein genes in response to pressure overload, which preceded the occurrence of hypertrophy and myocardial dysfunction. Thus, altered expression of Ca2+-handling protein genes may be one of the primary responses to pressure overload rather than a phenomenon secondary to myocardial hypertrophy.
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Abbreviations
- LV:
-
left ventricle
- DHP:
-
dihydropyridine
- SR:
-
sarcoplasmic reticulum
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Aoyagi, T., Fujii, A., Flanagan, M. et al. Maturation-Dependent Differences in Regulation of Sarcoplasmic Reticulum Ca2+ ATPase in Sheep Myocardium in Response to Pressure Overload: A Possible Mechanism for Maturation-Dependent Systolic and Diastolic Dysfunction. Pediatr Res 50, 246–253 (2001). https://doi.org/10.1203/00006450-200108000-00014
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DOI: https://doi.org/10.1203/00006450-200108000-00014
