Abstract
We previously reported that the frequency of TCRBV2 and TCRBV6S5-bearing T-cells was high in patients in the acute phase of Kawasaki disease (KD) and that streptococcal pyrogenic exotoxin C (SPE-C) was a potent stimulator of these TCRBV-bearing T-cells. To further elucidate the pathogenesis of KD, we examined the T-cell receptor (TCR) repertoire, human leukocyte antigen (HLA)-DRB1 genotype, and antibody responses to recombinant(r) SPE-C in patients with KD. We also performed in vitro stimulation with rSPE-A and rSPE-C of peripheral blood mononuclear cells from healthy donors and characterized the reacting T-cells. The percentage of T-cells bearing TCRBV2 and TCRBV6S5 was high in patients in the acute stage of KD. rSPE-C stimulation of PBMC from healthy donors induced expansion of TCRBV2 and TCRBV6S5-bearing T-cells. Furthermore, serum levels of anti-SPEC antibodies, which did not display antimitogenic activity, were higher in patients with acute KD than in age-matched controls. The frequencies of the DRB1*04051, 0406, and 0901 were high, whereas that of the DRB1*1101 was low among patients with KD as compared with the healthy adults.
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Abbreviations
- HLA:
-
human leukocyte antigen
- IVIg:
-
intravenous immunoglobulin
- KD:
-
Kawasaki disease
- SPE-A:
-
streptococcal pyrogenic exotoxin A
- SPE-C:
-
streptococcal pyrogenic exotoxin C
- SSOPs:
-
sequence-specific oligonucleotide probes
- TCR:
-
T-cell receptor
- TSS:
-
toxic shock syndrome
- SA:
-
superantigen
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Acknowledgements
We are grateful to Dr. Ichiro Kurane (Department of Virology 1, National Institute of Infection Disease, Tokyo, Japan) for the critical reading of the manuscript.
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Shionogi & Co., Ltd. and Wakayama Medical University supported this study.
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Yoshioka, T., Matsutani, T., Toyosaki-Maeda, T. et al. Relation of Streptococcal Pyrogenic Exotoxin C as a Causative Superantigen for Kawasaki Disease. Pediatr Res 53, 403–410 (2003). https://doi.org/10.1203/01.PDR.0000049668.54870.50
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DOI: https://doi.org/10.1203/01.PDR.0000049668.54870.50
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