Abstract
To evaluate effects of polymyxin B direct hemoperfusion (PMX-DHP) on a neonatal sepsis cecal ligation and perforation (CLP) model, in 24 anesthetized and mechanically ventilated 3-d-old piglets, 16 were assigned to CLP and an arteriovenous extracorporeal circuit from 3 h until 6 h post-CLP, with a PMX-column in PMX-DHP–treated group (8 piglets) and 8 as sham. Plasma lipopolysaccharide (LPS) was measured at before CLP and at 3 and 9 h. Changes in mean systemic blood pressure (mSBP), mean pulmonary blood pressure, serum IL-6, tumor necrosis factor alpha, interferon gamma, and highly mobile group-1 box protein were measured before CLP and at 1, 3, 6, and 9 h. LPS was lower in the sham and PMX-DHP groups than in the control at 9 h. The mSBP was higher in the sham and PMX-DHP groups than in the control at both 6 h. IL-6 was lower in the sham and PMX-DHP groups than in the control at 6 h. HMGB-1 was lower in the PMX-DHP group than in the control at 6 h. IFN-γ was only detected in the control group at 9 h. Survival times in the PMX-DHP group were longer than in the control. Thus, PMX-DHP improved septic shock in a neonatal septic model.
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Abbreviations
- CLP:
-
cecal ligation and perforation
- CVP:
-
central venous pressure
- HMGB-1:
-
highly mobile group-1 box protein
- IFN-γ:
-
interferon gamma
- LPS:
-
lipopolysaccharide (endotoxin)
- mPAP:
-
mean pulmonary blood pressure
- mSBP:
-
mean systemic blood pressure
- PMX-DHP:
-
polymyxin B direct hemoperfusion
- TNF-α:
-
tumor necrosis factor alpha
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Toray Industries (Tokyo, Japan) provided the polymyxin B immobilized fiber columns.
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Hussein, M., Kato, T., Sugiura, T. et al. Effect of Hemoperfusion Using Polymyxin B-Immobilized Fiber on IL-6, HMGB-1, and IFN Gamma in a Neonatal Sepsis Model. Pediatr Res 58, 309–314 (2005). https://doi.org/10.1203/01.PDR.0000169995.25333.F7
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DOI: https://doi.org/10.1203/01.PDR.0000169995.25333.F7
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