Abstract
We have recently shown that albumin added to meconium before intratracheal instillation in newborn pigs limits detrimental effect on the lungs and reduces increase of IL-8. The aim of this study was to test the effect of albumin instillation as rescue treatment in meconium aspiration syndrome (MAS). MAS was induced in hypoxic piglets by lung instillation of meconium (MAS I = 675 mg/kg, n = 12; MAS II = 540 mg/kg, n = 14). Morbidity and mortality differed (MAS I, dead = 7/12; MAS II, dead = 5/14). MAS groups were randomized to postmeconium instillation of either bovine albumin (30%, 1.4 mL/kg; MAS I, n = 6; MAS II, n = 7) or isotonic saline (9 mg/mL, 1.4 mL/kg; MAS I, n = 6; MAS II, n = 7). The controls (n = 4) were tested by sequential instillation of saline (9 mg/mL, 5 mL/kg) and albumin (30%, 1.4 mL/kg). Lung function and gas exchange deteriorated significantly after instillation of meconium [oxygenation index (OI): MAS I, +814%; MAS II, +386%; ventilation index (VI): MAS I, +256%; MAS II, +162%; compliance: MAS I, −53%; MAS II, −44%]. Increases of tracheal IL-8 correlated to deterioration of lung function were 10- (MAS I) and 5-fold (MAS II) (p < 0.001). Lung compliance was higher in albumin instillation versus saline instillation (MAS I, p = 0.008; MAS II, p = 0.002). Albumin did not influence intergroup differences in IL-8, hemodynamics, OI, or VI. MAS-induced IL-8 increases correlated with deterioration of lung function (OI, VI, and compliance). Rescue treatment with albumin in meconium aspiration improved lung compliance in piglets and may represent a new therapeutic approach to MAS.
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Abbreviations
- LAP:
-
left atrial pressure
- MAS:
-
meconium aspiration syndrome
- MAS I:
-
piglets receiving 675 mg meconium/kg
- MAS II:
-
piglets receiving 540 mg meconium/kg
- OI:
-
oxygenation index
- PAP:
-
pulmonary artery pressure
- VI:
-
ventilation index
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Acknowledgements
The authors thank Roger Odegaard for highly skilled assistance in surgery, and Hilde Nilsen and Anne Randi Alvestad for excellent laboratory assistance.
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Supported by The Research Council of Norway through a research fellowship for P.H.H.L.
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Lindenskov, P., Castellheim, A., Aamodt, G. et al. Meconium Induced IL-8 Production and Intratracheal Albumin Alleviated Lung Injury in Newborn Pigs. Pediatr Res 57, 371–377 (2005). https://doi.org/10.1203/01.PDR.0000153870.66197.D1
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DOI: https://doi.org/10.1203/01.PDR.0000153870.66197.D1
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