Abstract
Newborn resuscitation with pure oxygen may be associated with long-term detrimental effects. Due to the change in attitude toward use of less oxygen upon resuscitation, there is a need to study effects of intermediate hyperoxia. The aim was to study dose-response correlation between inspiratory fraction of oxygen used for resuscitation and urinary markers of oxidative damage to DNA and amino acids. Hypoxemia was induced in newborn piglets following a standardized model; they were resuscitated for 15 min with either 21%, 40%, 60% or 100% oxygen and observed for 1 h. Urine samples were collected. Urinary elimination of 8-hydroxy-2′-deoxyguanosine (8-oxo-dG), 2′deoxyguanosine (2dG), ortho-tyrosine (o-Tyr) and phenylalanine (Phe) were determined by HPLC and tandem mass spectrometry (HPLC-MS/MS). Quotient of 8-oxo-dG/2dG and o-Tyr/Phe ratios were significantly and dose-dependant higher in piglets resuscitated with supplementary oxygen. 8-oxodG/dG: Mean (SD) 5.76 (1.81) versus 22.44 (12.55) p < 0.01 and o-Tyr/Phe: 19.07 (10.7) versus 148.7 (59.8)for 21% versus 100%, p < 0.001. Hypoxia and subsequent resuscitation for 15 min with graded inspiratory fraction of oxygen causes increased oxidative stress and a dose-dependant oxidation of DNA and Phenylalanine. The increase in the hydroxyl attack may lead to a pro-oxidative status and risk for genetic instability.
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Abbreviations
- 8-oxodG:
-
8-hydroxy-2′-deoxyguanosine
- dG:
-
2′deoxyguanosine
- HPLC-MS/MS:
-
high-performance liquid chromatography and tandem mass spectrometry
- o-Tyr:
-
ortho-tyrosine
- Phe:
-
phenylalanine
- ROS:
-
reactive oxygen species
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Solberg, R., Andresen, J., Escrig, R. et al. Resuscitation of Hypoxic Newborn Piglets With Oxygen Induces a Dose-Dependent Increase in Markers of Oxidation. Pediatr Res 62, 559–563 (2007). https://doi.org/10.1203/PDR.0b013e318156e8aa
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DOI: https://doi.org/10.1203/PDR.0b013e318156e8aa
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