Abstract
Expression of IL-10 is decreased in lungs of preterm infants. We determined the constitutive and lipopolysaccharide (LPS)-induced IL-10 synthesis by lung inflammatory cells from preterm and term infants and examined their relationship to gestational age and/or incidence of bronchopulmonary dysplasia (BPD). A total of 37 infants; preterm neonates at gestational ages of 23–27 wk (group 1); 28–34 wk (group 2), and four full-term infants with meconium aspiration (group 3) were enrolled. One sample of lung inflammatory cells, obtained during postnatal d 1–3, and another during postnatal d 4–7 were cultured in vitro in presence or absence of 100 μg/mL of LPS. Secreted IL-10 was measured by ELISA. A positive relationship was found between gestational age and LPS-induced, but not constitutive IL-10 production within 1–3 d of life; group 1 on d 1–3 had a significant number of IL-10 nonresponders compared with group 2. All term neonates in group 3 had positive LPS-induced IL-10 response. Thus, in utero maturation of IL-10 gene expression is due to acquisition of inducibility. In contrast, constitutive IL-10 production within d 1–3 of life correlated with, and predicted the incidence of BPD in the highly vulnerable very premature infants.
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Abbreviations
- BPD:
-
bronchopulmonary dysplasia
- BW:
-
birth weight
- GA:
-
gestational age
- LPS:
-
lipopolysaccharides
- TAF:
-
tracheal aspirate fluid
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The authors thank the nurses, respiratory therapists, and other personnel of the neonatal Intensive Care Unit at LAC+USC Medical Center.
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Supported by the Hastings Foundation and National Institutes of Health, National Heart, Lung, and Blood Institute.
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Garingo, A., Tesoriero, L., Cayabyab, R. et al. Constitutive IL-10 Expression by Lung Inflammatory Cells and Risk for Bronchopulmonary Dysplasia. Pediatr Res 61, 197–202 (2007). https://doi.org/10.1203/pdr.0b013e31802d8a1c
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DOI: https://doi.org/10.1203/pdr.0b013e31802d8a1c
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