Abstract
Premature newborn baboons [125 d (67%) gestation], exposed to a moderate-size patent ductus arteriosus (PDA) [pulmonary-to-systemic blood-flow-ratio (Qp/Qs) = 1.8] for 14 d, have impaired pulmonary function and arrested alveolar development and surface area when compared with age matched fetuses (140 d gestation). Pharmacologic closure of the PDA reduces the detrimental effects of preterm delivery on pulmonary function and surface area. We used preterm baboons (delivered at 125 d gestation and ventilated for 14 d) to study the effects of surgical PDA ligation on pulmonary function and alveolar surface area. After ligation (on day of life 6), ligated animals had lower Qp/Qs ratios [Qp/Qs (ligated, n = 10) = 1.00 ± 0.04; (nonligated, n = 12) = 2.05 ± 0.17; mean ± SD] and higher systemic blood pressures than nonligated control animals. Ventilation and oxygenation indices did not differ between the groups, during either the pre- or postoperative periods. Alveolar surface area measurements were made by digital image analysis and compared with measurements made from fetal lungs at 125 d (n = 6) and 140 d (n = 7) gestation. PDA ligation failed to improve the postnatal arrest in alveolar surface area. In contrast with pharmacologic closure of the PDA, surgical closure failed to improve either pulmonary function or alveolar surface area in baboons with a moderate PDA shunt.
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Abbreviations
- BPD:
-
bronchopulmonary dysplasia
- PDA:
-
patent ductus arteriosus
- Qp/Qs:
-
pulmonary to systemic blood flow ratio
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Acknowledgements
We thank all the personnel who support the BPD Resource Center: the animal husbandry group led by Drs. D. Carey and M. Leland, the NICU staff (H. Martin, D. Correll, L. Kalisky, L. Nicley, R. Degan, S. Salazar, S. Ali), Deborah Catland, N.N.P., and the Wilford Hall Medical Center neonatal fellows who assist in the care of the animals, and the UTHSCSA pathology staff (L. Buchanan, K. Symank, Y. Valdes and K. Mendoza) who perform necropsies. Francoise Mauray performed the artwork. We would especially thank Vickie Winter, who has skillfully managed to categorize and keep track of all the tissue samples and animal data over the years and Dr. Jackie Coalson, who provided invaluable leadership, guidance, and scientific oversight for the BPD Resource Center and with whom we have had many thought provoking discussions.
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Supported in part by NIH Grants HL63399, HL52636 BPD Resource Center, P51RR13986 Primate Center facility support; HL63397 (to L.-Y.C.); HL46691, HL56061 and a gift from the Jamie and Bobby Gates Foundation (to R.I.C.).
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Chang, L., McCurnin, D., Yoder, B. et al. Ductus Arteriosus Ligation and Alveolar Growth in Preterm Baboons With a Patent Ductus Arteriosus. Pediatr Res 63, 299–302 (2008). https://doi.org/10.1203/PDR.0b013e318163a8e4
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DOI: https://doi.org/10.1203/PDR.0b013e318163a8e4
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