Abstract
Premature infants often develop serious clinical complications associated with respiratory failure and hyperoxic lung injury that includes lung inflammation and alterations in lung development. The goal of these studies is to test the hypothesis that there are differences in the course of lung injury in newborn mice exposed to 85% or >95% oxygen that provide models to address the differential effects of oxidation and inflammation. Our results indicate differences between the 85% and >95% O2 exposure groups by day 14 in weight gain and lung alveolarization. Inflammation, assessed by neutrophil counts, was observed in both hyperoxia groups by day 3 but was dramatically greater in the >95% O2-exposed groups by day 14 and associated with greater developmental deficits. Cytoplasmic phospholipase A2, cyclooxygenase-2, and 5-lipoxygenase levels were elevated but no patterns of differences were observed between exposure groups. Prostaglandins D2, E2, and F2α were increased in the tissues from mouse pups exposed to >95% O2 at 7 d indicating a differential expression of cyclooxygenase-2 products. Our data indicate that there are differences in the models of 85% or >95% O2 exposure and these differences may provide mechanistic insights into hyperoxic lung injury in an immature system.
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Abbreviations
- BPD:
-
bronchopulmonary dysplasia
- COX-2:
-
cyclooxygenase-2
- cPLA2:
-
cytoplasmic phospholipase A2
- LO:
-
lipoxygenase
- LT:
-
leukotriene
- PG:
-
prostaglandin
- RA:
-
room air;,
- TBX:
-
thromboxane
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Acknowledgements
We thank Xiaomei Meng, Molly Augustine, and Kathryn Heyob for their technical support.
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This work was supported by NHLBI, HL068948.
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Rogers, L., Tipple, T., Nelin, L. et al. Differential Responses in the Lungs of Newborn Mouse Pups Exposed to 85% or >95% Oxygen. Pediatr Res 65, 33–38 (2009). https://doi.org/10.1203/PDR.0b013e31818a1d0a
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DOI: https://doi.org/10.1203/PDR.0b013e31818a1d0a
