Abstract
Background:
In acute encephalopathy, deterioration of the condition can be rapid, and early intervention is essential to prevent progression of the disease. However, in the acute period, differentiating acute encephalopathy from febrile seizures is difficult. Thus, an early diagnostic marker has been sought to enable early intervention. Proton nuclear magnetic resonance (1H NMR) spectroscopy is used to study the chemical characteristics of biological fluids such as cerebrospinal fluid (CSF). The purpose of this study was to ascertain if pattern recognition of 1H NMR spectra could differentiate CSF obtained from patients with acute encephalopathy and febrile seizures.
Methods:
CSF was obtained from patients with acute encephalopathy (n = 4), complex febrile seizures (n = 9), and simple febrile seizures (n = 9).
Results:
NMR spectra of CSF did not visually differ across the three groups. Spectral data were analyzed by partial least squares discriminant analysis and visualized by plotting the partial least squares scores of each sample. The three patient groups clustered separately on the plots.
Conclusion:
In this preliminary study, we were able to visualize different characteristics of CSF obtained from patients with acute encephalopathy and simple and complex febrile seizures using pattern recognition analysis of 1H NMR data.
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Acknowledgements
The authors specially thank Kaori Okihara, Tomoko Konta, Hideyuki Morikawa, Hideki Muraki, and Tsuyoshi Moriyama for their work on sample preparation, acquisition of 1H NMR data, and support of data analysis to complete this research.
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Asano, T., Hirakawa, K., Koike, K. et al. Visualization of different characteristics of cerebrospinal fluid with acute encephalopathy and febrile seizures using pattern recognition analysis of 1H NMR. Pediatr Res 77, 70–74 (2015). https://doi.org/10.1038/pr.2014.141
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DOI: https://doi.org/10.1038/pr.2014.141
