Abstract
Background:
Congenital obstructive nephropathy (CON) is a leading cause of pediatric chronic kidney disease (CKD). Despite optimal surgical and medical care, there is a high rate of CKD progression. Better understanding of molecular and cellular changes is needed to facilitate development of improved biomarkers and novel therapeutic approaches in CON.
Methods:
The megabladder (mgb) mouse is an animal model of CKD with impaired bladder emptying, hydronephrosis, and progressive renal injury. In this study, we characterize a particular microRNA, miR-205, whose expression changes with the degree of hydronephrosis in the mgb−/− kidney.
Results:
Expression of miR-205 is progressively increased in the adult mgb−/− mouse with worsening severity of hydronephrosis. miR-205 expression is correlated with altered expression of cytokeratins and uroplakins, which are markers of cellular differentiation in urothelium. We describe the spatial pattern of miR-205 expression, including increased expression in renal urothelium and novel miR-205 expression in medullary collecting duct epithelium in the congenitally obstructed kidney.
Conclusion:
miR-205 is increased with severity of CON and CKD in the mgb−/− mouse and may regulate urothelial differentiation.
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The authors gratefully acknowledge the assistance of Jamie Mollett in the preparation of this manuscript.
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Wilhide, M., Feller, J., Li, B. et al. Renal epithelial miR-205 expression correlates with disease severity in a mouse model of congenital obstructive nephropathy. Pediatr Res 80, 602–609 (2016). https://doi.org/10.1038/pr.2016.121
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DOI: https://doi.org/10.1038/pr.2016.121
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