Fig. 3

mRNA analysis using reverse transcriptase PCR. Exon 4 skipping was analyzed by reverse transcriptase PCR. a Densitometry analysis of exon 4 skipping as represented by a microchip-based capillary electrophoresis system is shown. UM upper marker dye. b Sequencing of skeletal muscle and cardiomyocyte mRNA from the patient revealed skipping of exon 4. SM Pt skeletal muscle from the patient; CM Pt cardiomyocytes from the patient; SM Ctrl skeletal muscle from a healthy control