Fig. 3 | Journal of Human Genetics

Fig. 3

From: Identification of cytotoxic T cells and their T cell receptor sequences targeting COVID-19 using MHC class I-binding peptides

Fig. 3

Identification of HLA-A*02:01-binding peptides derived from SARS-CoV-2. A IFN-γ ELISPOT assay was performed after in vitro CTL induction. CD8+ T cells and HLA-A*02:01-positive T2 cells pulsed with each peptide were co-cultured overnight. T2 cells without the peptide were used as negative control. Bars represent the number of IFN-γ spots. CD8+ T cell responses were judged as positive when the number of IFN-γ spots were 1.5 times or higher than the negative control. B To confirm the response of CTLs to SARS-CoV-2-derived peptides, IFN-γ ELISA was performed. CTLs (Responders) were co-cultured overnight with T2 cells (Stimulators) pulsed with or without the peptide at the indicated ratio of Responders to Stimulators (R/S ratio). IFN-γ secretion was measured by ELISA. CTLs that revealed the peptide-specific IFN-γ secretion for eight peptides are shown. Similar results were obtained in independent experiments using three CTLs in the respective peptides

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