Fig. 4 | Journal of Human Genetics

Fig. 4

From: Identification of cytotoxic T cells and their T cell receptor sequences targeting COVID-19 using MHC class I-binding peptides

Fig. 4

Detection of CD8+ T cells that recognized SARS-CoV-2-derived MHC class I-binding peptides in PBMCs. A Gating strategy for detecting SARS-CoV-2-derived peptides-reactive CD8+ T cells. Firstly, lymphocytes population was separated by a forward scatter (FSC-A) and back scatter (BSC-A) gates. Doublets were excluded on forward scatter height (FSC-H) and forward scatter width (FSC-W). Living CD3+ CD4− cells were selected by staining with DAPI, and expression of CD3 and CD4. pMHC1 tetramer-binding CD8+ T cells were defined by gating based on HIV control tetramer staining. B HLA-A*24:02-positive PBMCs from three COVID-19-recovered individuals were stained using pMHCI tetramers after in vitro stimulation. CD8+ T cells that recognize SARS-CoV-2-derived peptides were detected from every individual. C HLA-A*24:02-positive PBMCs from three healthy individuals without any history of SARS-CoV-2 infection were stained using pMHCI tetramers after in vitro stimulation. Pre-existing CD8+ T cells that recognize SARS-CoV-2-derived peptides were detected from one of the three non-infected individuals

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