Fig. 2: PEX5 regulates the expression of peroxisomal genes during starvation.
From: PEX5 regulates autophagy via the mTORC1-TFEB axis during starvation
a Cells were treated with CTL and PEX5 siRNAs (100 nM) for 24 h. Then, cells were incubated in the presence or absence of serum. After 24 h, cells were stained with antibodies against PMP70 and catalase, and analyzed by fluorescence microscopy. Scale bar: 10 μm. b Cells were treated with CTL and PEX5 siRNAs for 24 h. Then, cells were incubated in the presence or absence of serum. After 24 h of incubation, RNA was extracted and Q-PCR analysis of peroxisomal genes was performed. Data are presented as mean ± s.d. from three independent experiments (**P < 0.01, *P < 0.05). c Transcript levels of PGC-1α and PPARα were measured by Q-PCR (*P < 0.05)
