Fig. 3: PEX5 depletion inhibits nuclear translocation of TFEB in response to starvation. | Experimental & Molecular Medicine

Fig. 3: PEX5 depletion inhibits nuclear translocation of TFEB in response to starvation.

From: PEX5 regulates autophagy via the mTORC1-TFEB axis during starvation

Fig. 3: PEX5 depletion inhibits nuclear translocation of TFEB in response to starvation.The alternative text for this image may have been generated using AI.

a Cells were treated with CTL and PEX5 siRNAs (100 nM) for 24 h. Then, cells were incubated in the presence or absence of serum. After 24 h, cells were stained with an antibody against TFEB and analyzed by fluorescence microscopy. Scale bar: 10 μm. b After HepG2 cells were treated as indicated, cells were subjected to nuclear and cytosol fractionation and immunoblotted with antibodies against TFEB, PCNA, and β-actin. PCNA and β-actin were used as nuclear and cytosolic markers, respectively. c, d Cells were transfected with CTL or TFEB siRNA (100 nM) for 24 h, and then incubated in the presence or absence of serum for 24 h. Protein expression of TFEB, LC3 and SQSTM1/p62 (autophagy proteins), and PMP70, DBP, and catalase (peroxisomal proteins) were measured by immunoblotting. e Q-PCR analysis of peroxisomal genes was performed (**P < 0.01, *P < 0.05)

Back to article page