Fig. 4: Knockdown of PEX5 inhibits lysosomal activity in serum-starved cells.

Cells were transfected with CTL siRNA (100 nM) and PEX5 siRNA (100 nM) for 24 h, and then incubated in the presence or absence of serum for 24 h. After incubation, cells were stained with LysoTracker Red DND-99 (50 nM) for 3 h and analyzed by confocal microscopy (a) or fluorescence-activated cell sorting and quantified (b). Data are presented as mean ± s.d. from three independent experiments (*P < 0.05). c Transcript levels of TFEB target genes were measured by Q-PCR. Values are expressed as fold increase compared to the starvation control group. Data were presented as mean ± s.d. from three independent experiments (**P < 0.01, *P < 0.05). d, e Protein expression of LAMP-1/LAMP-2 (lysosomal proteins) and cathepsins B/D (lysosomal proteases) were measured by immunoblotting