Fig. 8: Long-term HFD consumption reduces hepatic expression of glutaredoxins (Grxs). | Experimental & Molecular Medicine

Fig. 8: Long-term HFD consumption reduces hepatic expression of glutaredoxins (Grxs).

From: Glutathione disulfide sensitizes hepatocytes to TNFα-mediated cytotoxicity via IKK-β S-glutathionylation: a potential mechanism underlying non-alcoholic fatty liver disease

Fig. 8

Male C57BL/6 mice were fed with either a control diet or high-fat diet (HFD) for 12 weeks. a Hepatic expression of Grx1 and Grx2. b Cdcl2, a chemical inhibitor of Grx, increased protein-SSG formation in hepatocytes. HepG2 cells were incubated with Cdcl2 (1 μM) for 16 h. Whole-cell lysates were collected and subjected to western blot analysis for the detection of the intracellular protein-SSG levels. c Cdcl2 pretreatment sensitized hepatocytes to TNFα-induced cell death. HepG2 cells were pretreated with Cdcl2 (1 μM) for 2 h before TNFα (40 ng/mL) addition. Cell death was measured by LDH assay 16 h later. All values are expressed as the mean ± SD from three or more independent studies. *p < 0.01 versus untreated cells

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