Table 1 Efficiency of HDR-mediated KI mouse generation using non-overlapping or overlapping sgRNAs
From: Multiple sgRNAs with overlapping sequences enhance CRISPR/Cas9-mediated knock-in efficiency
Target locus | Method | KI fragment | KI length (bp) | sgRNAs | Embryos injected (n) | Embryo KI, n (%) | Pups delivered, n (%) | KI, n (%) | Related data |
|---|---|---|---|---|---|---|---|---|---|
Rosa26 | ssODN | loxP-MCS-loxP2272 | 110 | Overlapping | 92 | 8(9) | 3(38) | Fig. 1 | |
Upf1 | ssODN | loxp (intron 1) | 34 | Overlapping | 25 | 3/23 (13) | Fig. 2 | ||
Non-overlapping | 49 | 2/44 (4) | |||||||
Srebf1 | ssODN | loxP (intron 1) | 34 | Overlapping | 610 | 102 (17) | 18 (18) | Figure S1 | |
loxP (intron 4) | 34 | Non-overlapping | 8 (8) | ||||||
Tert | ssODN | loxP (intron 1) | 34 | Overlapping | 170 | 20(12) | 5 (25) | Figure S2 | |
loxP (intron 15) | 34 | Overlapping | 7 (35) | ||||||
Morc2a | ssODN | Morc2a (cC260T) | 1 | Overlapping | 36 | 12/27 (44) | Fig. 3 | ||
Non-overlapping | 118 | 1/89 (1) | |||||||
Adora2b | ssODN | Adora2b (cA890G) | 1 | Overlapping | 80 | 12 (15) | 3 (23) | Fig. 4 | |
Non-overlapping | 102 | 13 (13) | 1 (8) |
