Fig. 2: EN1 expression is regulated by the β-catenin/TCF complex through direct binding to the EN1 promoter region. | Experimental & Molecular Medicine

Fig. 2: EN1 expression is regulated by the β-catenin/TCF complex through direct binding to the EN1 promoter region.

From: Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells

Fig. 2

a Co-IP analysis showed that the interaction between β-catenin and TCF4 increased by BIO-treatment. b, c ChIP assays for β-catenin/TCF4 complex to the putative binding motif of EN1 promoter. The y-axis represents the means ± S.E.M. of the relative binding level of β-catenin (via TCF4) to each target sequence. c Expression of luciferase driven by a 5-kb-long regulatory region of human EN1 gene in HEK 293 cells. A reporter gene was strongly induced by over-expression of both constitutively active mutant of β-catenin (Cat4A) and WT-TCF4. d BIO treatment directly increased luciferase expression under the control of the EN1 regulatory sequence in human ESC-derived NPCs. Statistical significance was estimated using one-way ANOVA with multiple comparisons among groups from at least three independent experiments; * p < 0.05, ** p < 0.01.

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