Fig. 3: FGF signal is required for induction of EN1 by activation of Wnt signal. | Experimental & Molecular Medicine

Fig. 3: FGF signal is required for induction of EN1 by activation of Wnt signal.

From: Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells

Fig. 3

a Semi-qRT-PCR analysis showed endogenous and BIO-induced expression of various FGFs in human ESC-derived NPCs. b Inhibition of endogenous FGF signals by SU5402 significantly decreased basal EN1 expression. c Co-treatment with BIO and FGF8 slightly increased EN1 expression more than BIO alone whereas co-treatment of SU5402 with BIO abrogated the upregulation of EN1 expression (n = 4). d, e Immunoblotting corresponded with the EN1 expression in qRT-PCR (n = 3). f, g Nucleus localization of β-catenin was induced by Wnt activation, but not affected by FGF. White arrows indicate β-catenin in nucleus (scale bar: 20 μm, n = 3). Statistical significance was examined by one-way ANOVA; ††p = 0.001, *p < 0.05

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