Fig. 2 | Experimental & Molecular Medicine

Fig. 2

From: Hsa-let-7c controls the committed differentiation of IGF-1-treated mesenchymal stem cells derived from dental pulps by targeting IGF-1R via the MAPK pathways

Fig. 2

Identification of hsa-let-7c overexpression/knockdown in DPMSCs and effects of hsa-let-7c overexpression/knockdown on IGF-1R. a The hsa-let-7c binding site within IGF-1R, as predicted by TargetScan. b Selection of optimal transfection conditions for let-7c lentivirus transfection. MOI the ratio of the lentiviral concentration to the number of cells, V lentivirus, P polybrene, BF bright field, GFP green fluorescent protein. c The levels of hsa-let-7c gene expression in +Let-7c and +Ctrl DPMSCs. Values are presented as the mean ± SD, n = 3. *1 < 2−∆∆Ct < 2, P < 0.01. d The expression of IGF-1R protein in +Let-7c and +Ctrl DPMSCs, as determined by Western blot. e Quantitative analysis of d. **P < 0.01. f The levels of hsa-let-7c gene expression in −Let-7c and –Ctrl DPMSCs. Values are presented as the mean ± SD, n = 3. *1 < 2−∆∆Ct < 2, P < 0.01. g The expression of IGF-1R protein in –Let-7c and –Ctrl DPMSCs. h Quantitative analysis of g. **P < 0.01

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