Fig. 3 | Experimental & Molecular Medicine

Fig. 3

From: Hsa-let-7c controls the committed differentiation of IGF-1-treated mesenchymal stem cells derived from dental pulps by targeting IGF-1R via the MAPK pathways

Fig. 3

Effects of let-7c on the proliferation of IGF-1-treated DPMSCs. a, b CCK-8 assay revealed no significant differences between the growth curves of +Let-7c DPMSCs and +Ctrl DPMSCs (a) or between those of –Let-7c DPMSCs and –Ctrl DPMSCs (b). Values are presented as the mean ± SD, n = 3. c–f Flow cytometry was used to assess proliferative capabilities. The average proliferation index (PI = S + G2M) in +Ctrl DPMSCs (c), +Let-7c DPMSCs (d), –Ctrl DPMSCs (e), and –Let-7c DPMSCs (f) was 11.59, 10.34, 10.15, and 12.30%, respectively. g–j Flow cytometry was used to assess rates of cell apoptosis. The average rate of cell apoptosis in +Ctrl DPMSCs (g), +Let-7c DPMSCs (h), –Ctrl DPMSCs (i), and –Let-7c DPMSCs (j) was 6.40, 7.89, 6.66, and 8.64%, respectively

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