Fig. 5 | Experimental & Molecular Medicine

Fig. 5

From: Hsa-let-7c controls the committed differentiation of IGF-1-treated mesenchymal stem cells derived from dental pulps by targeting IGF-1R via the MAPK pathways

Fig. 5

Effects of let-7c on the mineralization ability of IGF-1-treated DPMSCs analyzed by Alizarin red staining. a Representative alizarin red staining images from +Let-7c, +Ctrl, −Let-7c, and –Ctrl DPMSCs cultured in α-MEM, α-MEM+IGF-1, mineralization media (MM), or MM+IGF-1 media. b Calcium nodules in different groups were visualized under an inverted microscope. c Calcium quantification demonstrated that the calcium content was significantly lower in +Let-7c DPMSCs compared to +Ctrl DPMSCs in the MM group. d The calcium content was significantly higher in −Let-7c DPMSCs compared to −Ctrl DPMSCs in both the MM and MM+IGF-1 groups. Values are presented as the mean ± SD, n = 3. **P < 0.01. Scale bars = 200 μm

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