Table 1 Sense and antisense primers for real-time RT-PCR
Gene | GenBank No. | Sequences (5′-3′) | Size (bp) |
|---|---|---|---|
OCN | NM_001199662.1 | Forward: AGCAAAGGTGCAGCCTTTGT | 63 |
Reverse: GCGCCTGGGTCTCTTCACT | |||
OSX | NM_152860.1 | Forward: CTCCTGCGACTGCCCTAA | 125 |
Reverse : GCGAAGCCTTGCCATACA | |||
DSPP | NM_014208.3 | Forward: ATATTGAGGGCTGGAATGGGGA | 136 |
Reverse: TTTGTGGCTCCAGCATTGTCA | |||
RUNX2 | NM_001024630.3 | Forward: TCTTAGAACAAATTCTGCCCTTT | 136 |
Reverse: TGCTTTGGTCTTGAAATCACA | |||
ALP | NM_000478.5 | Forward: GACCTCCTCGGAAGACACTC | 137 |
Reverse: TGAAGGGCTTCTTGTCTGTG | |||
COL-1 | NM_000090.3 | Forward: GAGCTGGCTACTTCTCGC | 103 |
Reverse: TCTATCCGCATAGGACTGAC | |||
DMP1 | NM_004407.3 | Forward: TTTTAGGAAGTCTCGCATCT | 209 |
Reverse: TGGGACCATCTACGTTTT | |||
GAPDH | NM_002046.3 | Forward: GAAGGTGAAGGTCGGAGTC | 225 |
Reverse: GAGATGGTGATGGGATTTC |
