Fig. 2: 13C metabolic flux analysis (13C-MFA) is a powerful approach for quantifying intracellular metabolic fluxes in cancer cells.
From: A guide to 13C metabolic flux analysis for the cancer biologist
The three inputs required for 13C-MFA are external uptake and secretion rates, isotopic labeling measurements, and a comprehensive compartmentalized model of cellular metabolism. User-friendly software tools for 13C-MFA, such as Metran and INCA, can be used to perform 13C-MFA calculations. These tools produce as outputs fluxes for all reactions in the model, confidence intervals for the estimated fluxes, and statistical analysis of the goodness-of-fit
