Fig. 6: MiR-192 regulated GLP1R expression. | Experimental & Molecular Medicine

Fig. 6: MiR-192 regulated GLP1R expression.

From: Exendin-4 ameliorates high glucose-induced fibrosis by inhibiting the secretion of miR-192 from injured renal tubular epithelial cells

Fig. 6

a The predicted miR-192-binding sites within the 3′-UTRs of GLP1R. (b,c) Relative expression of miR-192 in HK-2 cells transfected with miR-192 mimic or inhibitor. d, e Relative luciferase activity in HK-2 cells co-transfected with miR-192 mimic, inhibitor, or NC and pMIR-REPORT containing the GLP1R 3′-UTR site (*P < 0.05 versus the NC group). f, g Relative expression of GLP1R in HK-2 cells transfected with miR-192 mimic or inhibitor and exposed to HG (*P < 0.05 and **P < 0.01 versus the NC + NG group; #P < 0.05 and ##P < 0.01 versus the NC + HG group). h–k GLP1R expression in HK-2 cells transfected with miR-192 mimic or inhibitor and exposed to HG was analyzed by western blot (*P < 0.05 and **P < 0.01 versus the NC + NG group; #P < 0.05 and ##P < 0.01 versus the NC + HG group). l–n Relative expression of GLP1R in HK-2 cells after incubation with EVs derived from NG, HG, exendin-4, and Ex-HG groups for 48 h (*P < 0.05 versus the NG EV group; #P < 0.05 versus the HG EV group)

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