Fig. 1: Identification of MTB Rv-specific CD82 in mice and TB patients.

a Heat map of the top 13 upregulated genes in lungs from MTB Rv- or MTB Ra-infected mice. Each row shows the relative expression level of a single gene, and each column shows the expression level of a single sample. b Real-time qPCR analysis of CD82 expression or c bacterial loads in lungs from MTB Rv- or MTB Ra-intranasal infected mice for the indicated times (as in Fig. S1A). d Representative immunofluorescence images for expression of CD82 and CD68 (a macrophage marker) in lungs from MTB Rv- or MTB Ra-infected mice. Scale bar, 100 μM. The bottom panel shows the quantitative data of staining intensity of CD82 (left) and the colocalization index (%) between CD82 and CD68 (right). e Immunohistochemical analysis to examine CD82-DAB (3,3’-diaminobenzidine) and CD68-AEC (3-amino-9-ethylcarbazole) expression in healthy controls and patients with pulmonary TB. Representative images from five independent healthy controls and patients are shown. Insets, enlargement of outlined areas. Biological replicates (n = 3) for each condition were performed (a–d). Significant differences (**P < 0.01; ***P < 0.001) compared with MTB Ra (Student’s t-test with Bonferroni adjustment)