Fig. 5: DNMT inhibition induces CD82 protein expression and anti-mycobacterial responses in a CD82-dependent manner.

BMDMs were pretreated with 5-Aza (a for 10 μM and b–d for 5, 10, 20 μM) for 1 h and infected with MTB Rv or MTB Ra for the indicated times, followed by IB with αCD82 and αActin (a, b), culture supernatant harvesting at 18 h and analysis for cytokine ELISA for TNFα, IL-6, and IL-12p40 (c), or intracellular survival of MTB assessment by CFU assay (d). The data are representative of four independent experiments with similar results (a, b). Data shown are the means ± SD of five experiments (c, d). Significant differences (*P < 0.05; **P < 0.01; ***P < 0.001) compared with solvent control (Student’s t-test with Bonferroni adjustment). SC solvent control 0.1% DMSO, CFU colony-forming units