Table 1 Primers used in this study
From: CD82 hypomethylation is essential for tuberculosis pathogenesis via regulation of RUNX1-Rab5/22
Gene | Sense primer | Antisense primer | Size (bp) | Tm (°C) |
|---|---|---|---|---|
mCD82 genotyping | CAC CCT CCT GCA CTC AAT CT | CCA CCT GTG ACA ACC AAG TG | 612 | 60 |
mCD82 methylation 1 | GTT ATT GTT TTT CGT GTG AGA TTT C | ACC TTC CAT ATT ATT AAA ACA CCG A | 159 | 65.7 |
mCD82 Un-methylation 1 | TTA TTG TTT TTT GTG TGA GAT TTT GA | CCT TCC ATA TTA TTA AAA CAC CAA A | 157 | 65.2 |
mCD82 methylation 2 | TCG TAG ATT TAT TTT AGG GGT GTT C | AAT CTA CCT ATA TTT CCT TCC CGA C | 182 | 66.5 |
mCD82 Un-methylation 2 | TGT AGA TTT ATT TTA GGG GTG TTT GT | AAT CTA CCT ATA TTT CCT TCC CAA C | 181 | 66.1 |
mdnmt1 | GGG TCT CGT TCA GAG CTG | GCA GGA ATT CAT GCA GTA AG | 201 | 60 |
mdnmt2 | CCG CCT CTT CTT TGA GTT CTA C | AGA TGT CCC TCT TGT CAC TAA CG | 125 | 55 |
mdnmt3 | ATG GAG ATC AGG AGG GTA TGG A | GTC GCT TGG AGG TGG CTT TC | 177 | 56 |
mtet1 | GCA CCC CAA CC TAA TCA TC | ACC TTC ATT AGC TGC CTG GT | 172 | 58 |
mtet2 | ACA GAA GCA AGA ACA GCA GC | AGC TTG CAG GTG GAT TCT CT | 176 | 59 |
mtet3 | CCC CTT CCC ACT TCA CG AT | CAC AGC TTG TCT TGG AAC CC | 229 | 59 |
mβ-actin | AAG TGT GAC GTT GAC ATC CG | GAT CCA CAT CTG CTG GAA GG | 222 | 58 |
mCD82 ChIP-PCR | GCC ATT GTT TCC CGT GTG AGA CCT CG | TGC CTT CCA TGT TGT TGA GGC ACC GG | 160 | 58 |