Fig. 6: MID-00935 reduces fat mass and increases energy expenditure.

Four-week-old male mice were fed a high-fat diet for 8 weeks and orally treated with vehicle or MID-00935 (100 mpk, twice a day) for one week. After an overnight fast, gastrocnemius plus plantaris (Gp), soleus muscle, and liver weights were measured (a–c). Weights of Epididymal (EWAT), inguinal (IWAT), and brown adipose tissue (BAT) were measured (n = 12 for each group) (d–f). Four-week-old male mice were fed a high-fat diet for 10 weeks and orally treated with vehicle or MID-00935 (100 mpk, twice a day) for 2 weeks. After treating DIO mice with vehicle or MID-00935 for 1 week, food uptake, locomotor activity, V_O2, V_CO2, and energy expenditure were measured for 24 h (n = 10 for each group) (g–l). Core body temperatures were measured by a thermocouple thermometer with a rectal probe (m). Data are presented as the means ± SEM; *p < 0.05 and ***p < 0.001, compared to the vehicle-treated group