Fig. 1: EGF enhances the hnRNP Q1- mediated increase in Aurora-A mRNA translation.
From: The EGF/hnRNP Q1 axis is involved in tumorigenesis via the regulation of cell cycle-related genes
a SW480 cells were transfected with GFP or GFP-hnRNP Q1 and were incubated for 24 h. After serum starvation, cells were treated with EGF (10 nM) for 2 h, and total cell lysates were then harvested for western blot analysis. After EGF treatment, EGFR underwent endocytosis and ubiquitination31,32,33. b The levels of Aurora-A mRNA in GFP- and GFP-hnRNP-Q1-expressing SW480 cells were quantified following treatment with EGF. c Cell lysates were harvested for the ribosomal S6-IP assay. The amount of Aurora-A mRNA in the S6-IP complex was analyzed using RT-qPCR. The mean ± S.D. was obtained from three independent experiments (*p < 0.05; **p < 0.01; ***p < 0.001). d The expression level of the Aurora-A protein in hnRNP Q siRNA-transfected SW480 cells treated with EGF was determined by western blot analysis. GAPDH was used as the loading control. The expression level of Aurora-A is shown as a ratio
