Fig. 2: Hormone and growth factor upregulation of ARD1 in cancer.

a MCF-7 and LNCaP cells were serum starved for 48 and 72 h, respectively, and subsequently re-stimulated with 10% FBS for 48 h. ARD1 expression increased with time exposed to serum treatment. b MCF-7 cells were treated with increasing concentrations of β-estradiol (estrogen) for short and long time exposures. ARD1 expression increased with increasing both treatment duration and concentration of estrogen. N = 3, representative blots shown. Whole-cell lysates were collected and run on gradient 4–20% SDS gels and bands were detected via the ECL method