Fig. 3: PPP2CA dissociates from HDAC2 in response to hypertrophic stimuli.
From: PP2A negatively regulates the hypertrophic response by dephosphorylating HDAC2 S394 in the heart
The binding of PPP2CA to HDAC2 was reduced by hypertrophic stresses (a–c). In cardiomyocytes stimulated with 20 μmol/L PE, the interaction between HDAC2 and PPP2CA was decreased (a). Cardiac hypertrophy induced either by aortic banding (AoB) (b) or by isoproterenol (ISP, 30 mg/kg/day), a beta-adrenergic agonist, also provoked the dissociation of PPP2CA from HDAC2 (c). d To assess whether the hypertrophic stress-induced dissociation is HDAC2 phosphorylation-dependent CK2α1, an HDAC2 kinase was added to the immunoprecipitation assay. HDAC2 phosphorylation also detached PPP2CA from HDAC2. Data are presented as the mean ± SEM. **p < 0.01. White numbers in each bar denote the independent set used for densitometer analysis
