Fig. 2: Mitochondrial localization of CAMP-hMT1A and CAMP-EGFP by transfection. | Experimental & Molecular Medicine

Fig. 2: Mitochondrial localization of CAMP-hMT1A and CAMP-EGFP by transfection.

From: Cell-penetrating artificial mitochondria-targeting peptide-conjugated metallothionein 1A alleviates mitochondrial damage in Parkinson’s disease models

Fig. 2

SK-Hep1 cells were stably transfected with pcDNA3.1-myc/His (mock), CAMP-hMT1A-6xHis, EGFP, or CAMP-EGFP. a Confocal microscopy images showing the localization of CAMP fusion proteins. Cells were stained with MitoTracker (MitoT, red) and immunostained with anti-6xHis or EGFP antibodies (green) before microscopy (×400, scale bar = 10 μm). Co-localization of red and green signal appears yellow in merged images. b Western blot analysis. Cell or mitochondrial lysates were analyzed by western blot with 6xHis-tag to detect CAMP-hMT1A and β-actin and HSP60 antibodies for loading and mitochondria controls, respectively

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