Fig. 3: Preparation of CAMP-hMT1A recombinant protein. | Experimental & Molecular Medicine

Fig. 3: Preparation of CAMP-hMT1A recombinant protein.

From: Cell-penetrating artificial mitochondria-targeting peptide-conjugated metallothionein 1A alleviates mitochondrial damage in Parkinson’s disease models

Fig. 3

CAMP-hMT1A protein was expressed in E. coli in the presence of 1 mM ZnSO4 and purified by Ni-NTA affinity chromatography. a Coomassie blue staining. M molecular weight marker. b Western blot with 6xHis-tag and metallothionein (MT1A) antibodies to detect CAMP-hMT1A. c Mass spectrum analysis. The spectrum of the identified peptide sequence is presented

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