Fig. 4: Transfer of CAMP-hMT1A protein into mitochondria. | Experimental & Molecular Medicine

Fig. 4: Transfer of CAMP-hMT1A protein into mitochondria.

From: Cell-penetrating artificial mitochondria-targeting peptide-conjugated metallothionein 1A alleviates mitochondrial damage in Parkinson’s disease models

Fig. 4

a Western blot of TAT-hMT1A, CAMP-hMT1A, and CAMP-EGFP recombinant proteins using 6xHis-tag antibody. b Time-dependent uptake of recombinant proteins. SK-Hep1 cells expressing DsRed2-mito (red) were treated with 2 μM CAMP-hMT1A, TAT-hMT1A, or CAMP-EGFP for 3, 24, or 48 h. Cells were immunostained with 6xHis-tag or EGFP antibodies (green) before confocal microscopy (×400, scale bar = 10 μm). The yellow merged images show mitochondrial localization of the fusion proteins. c Western blot of CAMP-hMT1A in mitochondria isolated from cells treated with 2 μM CAMP-hMT1A for 24 h. The upper and lower bands of CAMP-hMT1A represent the precursor and mature forms, respectively. β-Actin and HSP60 antibodies were used for loading and mitochondria control, respectively. d Schematic representation of the precursor and mature forms of CAMP-hMT1A. The processing site is indicated by an arrow

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