Fig. 1: Generation and characterization of 5-FU-resistant cells from HCT-8 cells.
From: Wnt pathway is involved in 5-FU drug resistance of colorectal cancer cells
a Growth curve of HCT-8R and HCT-8 cells under 5-FU treatment. The data represent the means ± SD from three independent experiments. b Cell morphology of HCT-8R and HCT-8 cells (×400). Scale bar, 100 µm. c The result of FACS analysis shows the subG1 peak of HCT-8R and HCT-8 cells under treatment with 5 µg/mL 5-FU for 24 h. The FACS result shows the apoptotic ratio of cells induced by 5-FU treatment. HCT-8 WT control cell and HCT-8R resistant cell ratios were 8.1% and 0.2%, respectively. The data represent the means ± SD from three independent experiments. *P < 0.05. d TUNEL assay shows the apoptotic HCT-8 cells under 10 µg/mL 5-FU treatment for 48 h. Scale bar, 10 µm. The data represent the means ± SD from three independent experiments. **P < 0.01
