Fig. 3: Enhanced resistance to 5-FU is due to the suppressed checkpoint pathway.
From: Wnt pathway is involved in 5-FU drug resistance of colorectal cancer cells
a The knockdown efficiency of CHK1 was shown by immunoblot assay. b Overexpressed CHK1 expression was confirmed by immunoblotting assay. c The cell survival curve shows that the cell survival rate of the CHK1 knockdown HCT-8 cells with 10 µg/ml 5-FU was significantly higher than that of mock-transfected cells. d Overexpressed CHK1 expression decreased the cell survival rate in 10 µg/mL 5-FU-containing medium. e Cell morphology under a microscope. All cells transfected with CHK1 siRNA were treated with 10 µg/mL 5-FU for 48 h. Scale bar, 50 µm. f Cell morphology of CHK1-overexpressing HCT-8 cells and mock-treated cells (×400). Scale bar, 50 µm. g TUNEL assay shows the apoptotic cells induced by 5-FU treatment. Scale bar, 10 µm. h TUNEL assay shows the apoptotic cells induced by CHK1 overexpression. Scale bar, 10 µm
