Fig. 6: Histone modification is involved in Wnt-mediated regulation of CHK1.
From: Wnt pathway is involved in 5-FU drug resistance of colorectal cancer cells
a Histone acetylation status was different in HCT-8 and HCT-8R cells. Shown are the western blotting assays of H3K27ac and H3K14ac expression level. b TSA, a well-established histone deacetylase (HDAC) inhibitor, sensitized HCT-8R cells to 5-FU treatment. c Western blotting assay of HCT-8R cells treated with TSA. d Western blotting assay of HCT-8R cells treated with the Wnt signal inhibitor IWP-2. e CHK1 mRNA level in HCT-8R cells treated with IWP-2 or TSA. *p < 0.05. f Cell survival of HCT-8 cells treated with Wnt3a or TSA. **p < 0.01
