Fig. 1: Effect of XBP1s on insulin-stimulated glucose uptake in 3T3-L1 adipocytes. | Experimental & Molecular Medicine

Fig. 1: Effect of XBP1s on insulin-stimulated glucose uptake in 3T3-L1 adipocytes.

From: The IRE1α-XBP1s pathway promotes insulin-stimulated glucose uptake in adipocytes by increasing PPARγ activity

Fig. 1

3T3-L1 adipocytes were transfected with pcDNA3.1-mXBP1s (XBP1s) or an siRNA against XBP1 (siXBP1), and treated with insulin (1 μg ml−1) for 30 min as indicated. a, b Intracellular XBP1s mRNA and protein levels and glucose uptake rates were determined. c The expression (left) and translocation (right, green) of GLUT4 were analyzed using real-time PCR/immunoblot analyses and immunofluorescence, respectively. DAPI was used to stain nuclei (right, blue). d The total and phosphorylated expression levels of insulin receptor (IR), insulin receptor substrate 1 (IRS1), and AKT were examined by immunoblot analysis (left) and quantified with densitometry (right). Values are presented as the means ± SEM of three independent experiments. *P < 0.05 compared to empty vector (EV) or control siRNA (siCon); #P < 0.05 compared to insulin-treated EV. n.s. not significant

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