Table 1 Primer sequences for PCR analysis
Name | Primer sequences (5'–3') | Size (bp) | Gene ID | |
|---|---|---|---|---|
Sense | Antisense | |||
Adiponectin | TGTGTATCGCTCAGCGTTCAGTGT | AGAGAACGGCCTTGTCCTTCTTGA | 224 | NM_009605 |
aP2 | GAT GCC TTT GTG GGA ACC T | CTG TCG TCT GCG GTG ATT T | 230 | NM_024406 |
CD36 | CAG GTC TAT CTA CGC TGT GTT C | CAG GCT TTC CTT CTT TGC ATT T | 200 | NM_007643 |
ERdj4 | AGT CTG CCT CAG AGC GAC AAA TCA | GCC TCT TTG TCC TTT GCC ATT GGT | 203 | NM_013760 |
FGF21 | CTACACAGATGACGACCAAGA | ATCAAAGTGAGGCGATCCATAG | 205 | NM_020013 |
GLUT4 | TCG TGG CCA TAT TTG GCT TTG TGG | TAA GGA CCC ATA GCA TCC GCA ACA | 190 | NM_009204 |
Leptin | GGC TTT GGT CCT ATC TGT CTT AT | ATT CTC CAG GTC ATT GGC TAT C | 278 | NM_008493 |
LPL | ACGAGCGCTC CATTCATCTC TTCA | TCTTGCTGCT TCTCTTGGCT CTGA | 180 | NM_008509 |
MCP1 | GAA GGA ATG GGT CCA GAC ATA C | TCA CAC TGG TCA CTC CTA CA | 200 | NM_011333 |
p58IPK | TTT CAC GCT GCA GTT GAT GGT GAC | TCT TCT GCT TCG TCA AGC TTC CCT | 200 | NM_008929 |
PPARγt | CGA GCC CTG GCA AAG CAT TTG TAT | AAA TTC GGA TGG CCA CCT CTT TGC | 211 | NM_001308354 |
PPARγ2 | CGCTGATGCACTGCCTATGA | AGAGGTCCACAGAGCTGATTCC | 101 | NM_011146 |
TNF | CGTCAGCCGATTTGCTATCT | AGCAATGACTCCAAAGTAGACC | 232 | NM_013693 |
XBP1t | AGC AGC AAG TGG TGG ATT TGG AAG | AAG AGG CAA CAG TGT CAG AGT CCA | 299 | NM_013842 |
XBP1s | CTGAGGTCCGCAGCAGGT | TGTCAGAGTCCATGGGAAGA | 66 | NM_001271730 |
36B4 | GCTCCAAGCAGATGCAGCA | CCGGATGTGAGGCAGCAG | 143 | NM_007475 |
fgf21 promoter (ChIP assay) | CTC AGA CCA AGG AGC ACA GA | TGA ACG CAG AAA TAC CAG AAT | 257 | ENSMUST00000033099 |
pEGFP-mXBP1 | TTG AAT TCT GAT GGT GGT GGT GGC AGC GGC GCC GA | TTG TCG ACA GGC CTG CAC CTG CTG CAG AGG TGC ACA | – | – |
pcDNA-WT-mXBP1u | TTG CGG CCG CAT GGT GGT GGT GGC A | TTA AGC TTG ACA CTA ATC AGC TGG GGG AAA AGT TC | – | – |
pcDNA-Mut-mXBP1u | ACT CAG ACT ATG TGC ACC TTT GCG ACA GGT GCA GGC CCA GTT | AAC TGG GCC TGC ACC TGT CGC AAA GGT GCA CAT AGT CTG AGT | – | – |
