Fig. 5: MBNL1-AS1 targeted and negatively regulated KCNMA1.

a The predicted consequential pairing between MBNL1-AS1 and KCNMA1. b Relative luciferase activity in the NC, MBNL1-AS1 vector, and siRNA-MBNL1-AS1 groups determined by the dual-luciferase reporter gene assay. c RIP results in each group. MBNL1-AS1 muscleblind-like 1 antisense RNA 1, KCNMA1 potassium calcium-activated channel subfamily M alpha 1, si small interfering, NC negative control; *p < 0.05 vs. the NC group; measurement data are expressed as the mean ± standard deviation. Comparisons among multiple groups were performed by one-way ANOVA; the experiment was repeated 3 times