Table 3 Apoptosis rate and cell cycle distribution of skeletal muscle cells among seven groups

From: RETRACTED ARTICLE: Downregulation of the long noncoding RNA MBNL1-AS1 protects sevoflurane-pretreated mice against ischemia-reperfusion injury by targeting KCNMA1

Group

Apoptosis rate (%)

G0/G1 (%)

S (%)

G2/M (%)

Control

6.45 ± 0.51

71.35 ± 5.12

18.84 ± 1.13

9.81 ± 0.81

Blank

3.36 ± 0.32*

53.37 ± 3.27

35.72 ± 2.41

10.91 ± 1.02

NC

3.47 ± 0.31*

53.61 ± 3.62

34.67 ± 2.71

11.72 ± 1.11

MBNL1AS1 vector

5.21 ± 0.49*#

62.94 ± 4.71*#

27.14 ± 1.73*#

9.92 ± 0.93

si-MBNL1AS1#1

1.28 ± 0.11*#

45.42 ± 3.84*#

43.25 ± 3.61*#

11.33 ± 1.12

si-MBNL1AS1#2

1.22 ± 0.23*#

43.33 ± 2.47*#

43.33 ± 2.47*#

43.33 ± 2.47*#

si-KCNMA1#1

5.33 ± 0.48*#

63.16 ± 5.12

26.33 ± 1.73

10.51 ± 1.01

si-KCNMA1#2

4.97 + 1.1*#

61.89 ± 3.44

25.08 ± 1.23

10.34 ± 1.45

si-MBNL1AS1 + si-KCNMA1

3.42 ± 1.31*

58.72 ± 4.22

31.15 ± 2.63

10.13 ± 1.03

  1. MBNL1-AS1 muscleblind-like 1 antisense RNA 1, KCNMA1 potassium calcium-activated channel subfamily M alpha 1, si small interfering, NC negative control
  2. *p < 0.05 vs. the control group; # p < 0.05 vs. the blank and NC groups; measurement data were expressed as mean ± standard deviation and comparisons among multiple groups were assessed by one-way ANOVA; the experiment was repeated for 3 times