Fig. 4: Anti-metastatic effect of BJ-2302, a non-cytotoxic CTSS inhibitor, in an MDA-MB-231-luc-2-tdTomato cell-xenografted CAM model.

MDA-MB-231-luc-2-tdTomato cancer cells were inoculated at the top of the CAM in the presence of indicated drugs. a–d On the 5th day of drug treatment, tumor growth was visualized with images captured under the stereomicroscope (a), and the number of blood vessels within the tumor area and tumor weights (b) were measured. c, d Images of the developing lungs, liver, lower CAM, and upper CAM were captured for the detection of red-fluorescence-labeled cancer cells (c), and the tissues were examined for HPRT mRNA in the chick lungs and liver (upper bar) and the upper and lower parts of the CAM (lower bar) (d). The bar-diagram represents quantitative data. *P < 0.05 compared to vehicle-treated groups